NFKB1 and melanoma: Combination fluorescence and bioluminescence intravital microscopy was demonstrated using skin window chamber-bearing animals injected with B16F10 melanoma cells stably expressing a concatenated κB5 promoter-driven Firefly luciferase reporter (κB5-FLuc) [30,36] that monitors NF-κB activation and a constitutively active IRES promoter-driven GFP fluorescent reporter.