There were no changes in total CD8+ T cells in the tumor (Figure 4D), but there were significant increases in multifunctional CD8+ T cells (CD8+IFN-γ+GzmB+, Figure 4E) and CD8+ T cells with a proliferative capacity (CD8+Ki67+, Figure 4F) following PRGN-2009 treatment compared with empty vector control. This evidence concerns the gene IFNG and neoplasm.