Further study limitations include the lack of high-dimensional immunohistochemistry markers to delineate more subtypes of TILs21 beyond CD4/CD8/FOXP3, other key cell types such as macrophages, B cells20 and cancer-associated fibroblasts in the tumour ecosystem, as well as the use of 2D slides that do not fully represent the 3D ductal structure. This evidence concerns the gene FOXP3 and neoplasm.