Considering the constitutive expression of NLRP3 in melanoma cell lines, mice were implanted subcutaneously with the murine melanoma cells line B16F10 and compared to mice injected with B16F10 cells where NLRP3 was deleted (B16F10 nlrp3−/−) (SI Appendix, Fig. S3A). The gene discussed is NLRP3; the disease is melanoma.