Early on in the infection (1 dpi), numerous KEGG pathways that were most extremely triggered in infected diaphragms were connected with immune responses, specifically cytokine–cytokine receptor interactions and many signaling pathways, including the tumor necrosis factor, nuclear factor-kappa B, nodulation (NOD)-like receptor, mitogen-activated protein kinase (MAPK), Jak-STAT, HIF-1 and adipocytokine ones. This evidence concerns the gene TNF and infection.