ANOVA (see Table 3) revealed that neuropeptide content is markedly altered in obesity, with changes in the expression of α-MSH (α-Melanocyte-stimulating hormone, p = 0.0397), neurotensin (p = 0.0018), and melanin-concentrating hormone (MCH, p = 0.0003). This evidence concerns the gene NPS and obesity due to melanocortin 4 receptor deficiency.