To detect the optimal action concentration and time of IAV, p-NHBE cells were infected with IAV (MOI 0, 3, 6, 12, 25, or 50) for 24 h, and infected with IAV (MOI 50) for 1, 3, 6, 12, or 24 h, the results showed that the inhibition of E-cad and ZO-1 expression was the most obvious 24 h after IAV (MOI 50) infection (P < 0.05; Figure 1). This evidence concerns the gene CDH1 and infection.