Also, when GBM cells were 3D bioprinted in this biomaterial ink, a higher resistance to temozolomide was obtained, in comparison to that of 2D monolayered models.[227] 3D bioprinted alginate/gelatin hydrogels can also be employed for evaluating the invasiveness of cancer cells.[223, 224, 228] For instance, alginate/Matrigel/gelatin hydrogels were used to test the effect of transforming growth factor‐beta (TGF‐β)‐mediated HeLa cells migration, and when hydrogels were exposed to these molecules, cells forming 3D spheroids exhibited EMT and upregulated mesenchymal markers expression.[228]. Here, TGFB1 is linked to glioblastoma.