Although the proportion of activated (granzyme B+) CD8+ T cells was increased after anti‐PD1 antibody treatment (Figure 6B), even without a significant change in total CD8+ T cell count in both groups, a decreased absolute number of CD8+ T cell in the intratumoral region of the mCXCL12 overexpressing MC38 tumor may be insufficient to show the antitumorigenic effect of ICI. This evidence concerns the gene GZMB and neoplasm.