Diabetes caused increased testicular MDA content, miR-34a expression, acetylated p53 protein expression, and the percent of immotile sperm (P<0.01 to P<0.001) as well as reduced testicular GPx, SOD and catalase activities, SIRT1 protein expression, and sperm parameters (P<0.05 to P<0.001). The gene discussed is SIRT1; the disease is diabetes mellitus.