In addition, enforced expression of TMEM52B isoforms in HCT-116 cells, which display little to no TMEM52B expression, reduced phosphorylation of EGFR, AKT, JNK, ERK1/2, and c-Jun along with reduced cancer cell invasion and survival (Supplementary Fig. S5), confirming the effects of the TMEM52B isoforms. This evidence concerns the gene AKT1 and cancer.