In the analysis of unadjusted effect, there was no significant association between tPA (compared to placebo) and excellent functional outcome (RR = 1.05, 95% CI: 0.93–1.18, I2: 74%; Supplemental Fig. 3) or favorable functional outcome (RR = 1.06, 95% CI: 0.97–1.15, I2: 81%; Supplemental Fig. 4) in patients with SVD-associated ischemic stroke. The gene discussed is PLAT; the disease is ischemic stroke.