To confirm that Sirt1 is the target of lncRNA Sirt1-AS in the process of DVT, we detected the expression of lncRNA Sirt1-AS after the transfection of lncRNA Sirt1-AS shRNA or pcDNA vector (lncRNA Sirt1-AS) in normal or replicative senescence HUVECs. The gene discussed is SIRT1; the disease is deep vein thrombosis.