Moreover, silencing HIFAL expression abolished the hypoxia-induced transcriptional activity of HIFAL promoter as determined by luciferase reporter assays (Fig. 5k, l), while enforced expression of HIFAL in the breast cancer cells increased the transcriptional activity of HIFAL promoter (Fig. 5m). The gene discussed is ENSG00000258667; the disease is breast carcinoma.