As was indicated in the above data, the dynamics of HIF-1α protein level did not coincide with the expression of HIF-1 target genes (Fig. 1a), suggesting that HIF-1 effects become less dependent on HIF-1α level during prolonged hypoxia, which may limit the therapeutic effect of HIF-1α inhibitor as a single agent in cancer treatment. The gene discussed is HIF1A; the disease is cancer.