To understand the roles of CBP and Ku70 in human melanoma cells, we designed and synthesized a set of CBP siRNA and Ku70 siRNA, respectively, and examined their efficiencies in knocking down the CBP mRNA and the Ku70 mRNA in human melanoma A375 cell line by real-time quantitative polymerase chain reaction (PCR). Here, CREBBP is linked to melanoma.