Knockdown of SHMT2 attenuated the growth of prostate cancer cells (LNCaP, C4-2B, DU145, and PC3 cells) as well as various cancer cell lines including glioblastoma line LN229 and colon cancer cell lines HCT116 and SW480 (Fig. 6A) following gene silencing by siRNA (Fig. S11). This evidence concerns the gene SHMT2 and colonic neoplasm.