In consideration of these results totally, we thus selected AsPC-1 and Capan-2 cells as pancreatic cancer cell lines strongly expressing mesothelin (mesothelin-high), and Panc-1 and MIA Paca-2 cells as cell lines expressing low levels of mesothelin (mesothelin-low) for subsequent in vitro experiments. The gene discussed is MSLN; the disease is pancreatic neoplasm.