IRF3 and infection: As expected, in G3BP1/2 knock out cells, the levels of phospho-TBK1 and phospho-IRF3, triggered by rIBV-nsp15-H238A infection, were greatly decreased (Fig 10B); consequently, the transcription of IFN-β and ISG IFIT-1 induced by rIBV-nsp15-H238A infection were significantly decreased (Fig 10C).