ZNF639 and infection: To confirm the effects of ZASC1 on the HIV-1 promoter outside of infection and to confirm that the phenotype of our knockout cells is primarily due to deletion of ZASC1, we transiently transfected Jurkat and Jurkat ΔZASC1 cells with a plasmid containing the HIV-1 LTR promoter driving expression of Gaussia luciferase (HIV-gLuc).