ZBTB2 and viral infectious disease: To determine if ZBTB2 affected HIV-1 gene expression in the context of viral infection, we performed siRNA-mediated ZBTB2 depletion in the SupT1 T-cell line and then challenged the siRNA treated cells with a VSV-G pseudotyped HIV-1 vector with nonsense mutations in the envelope and Vpr genes and firefly luciferase inserted into the Nef locus.