Our findings revealed that high-risk scores were markedly associated with various cancer-related pathways, like hypoxia, TNFα signaling pathway via NF-κB, inflammatory response, mitotic spindle, PI3K-Akt-mTOR, Notch, interferon α response, interferon γ response and glycolysis pathways, indicating that cancer-related pathways were activated in glioma samples with high-risk scores. This evidence concerns the gene TNF and central nervous system cancer.