Further characterisation of the circulating immune cell landscape led to the identification of decreased levels of total CD3+ cells, CD8+ T cells, CD56dim NK cells, NKG2D+ NK cells, NKG2D+ CD56dim NK cells, NKG2D+ iNKT cells, PD1+CD4+ T cells, CXCR3+CD4+ T cells, PD1+CD25+CD45RA+CD4+ T-cells, and mucosal-associated invariant T (MAIT) cells, and elevated numbers of total CD4+ T cells, CD25+CD45RA+CD4+ T-cells and Th2 cells, when NAFLD patients were compared to healthy controls (162, 163). This evidence concerns the gene CD8A and metabolic dysfunction-associated steatotic liver disease.