NOS3 and fatty liver disease: Moreover, elevated hepatic ROCK1 activity has consistently been observed in a plethora of models of disordered metabolism including: humans with fatty liver disease (14), DIO mice (14), db/db mice (14), ob/ob mice (14), TNFα-treated hepatocytes (15), endothelial nitric oxide synthase (eNOS) deficient mice (16), palmitate-treated hepG2 cells (21), palmitate metabolite lysophosphatidylcholine (LPS) -treated Huh7 cells (20), LPS-treated mice (15), and DIO streptozotocin-treated rats (18).