UPP1 and androgen insensitivity syndrome: Based on the ROC curve, the optimal cutoff value of TF-UP/LRP ratio as a biomarker for the presence of AIS was projected to be 0.6317 and the area under the curve (AUC) was 0.9565, which yielded a sensitivity of 91.49% and a specificity of 88.57% (Figure 1A and Table 3).