Of note, at the concentrations (0.2–1 μM) and time (24 h) used, 4HW alone or in the presence of TNF-α reduced the cell viability by up to 16.9% in H1299 cells and 31.5% in A549 cells, suggesting that the inhibitory effect of 4HW on TF-mediated procoagulant activity was not mainly due to its cytotoxicity toward cancer cells (Fig. 2C). The gene discussed is TNF; the disease is cancer.