Supplementation of C3 recombinant protein in the conditioned medium of wild type HCC cells evoked its ability in inducing the population of CD11b + Ly6G + Ly6C- MDSCs (Supplementary Fig. S6c), with activation of its intracellular P38 MAPK signaling (Supplementary Fig. S6d) and IL10 production (Supplementary Fig. S6e). The gene discussed is IL10; the disease is hepatocellular carcinoma.