The lipoplexes with a cationic lipid: DOPE (helper lipid): mRNA ratio of 1.3:2 (≈250 nm in size and ≈30 mV in zeta potential) were shown to efficiently target the splenic DCs in mice and led to strong activation of NK, B, CD4+, CD8+ T cells, subsequently resulting in potent immunotherapeutic efficacy in multiple mouse cancer models and was translated into clinics. Here, CD8A is linked to cancer.