ANXA5 and breast cancer: In addition, the trypan blue staining assay results showed that 3-MA inhibited cell viability in DR-BC cells treated with high-dose doxorubicin (Fig. 3e), which were supported by the following Annexin V-FITC/PI double staining assay results and showed that 3-MA increased apoptosis ratio in high-dose doxorubicin stimulated DR-BC cells (Fig. 3f, g).