To identify oxysterol ligands that could promote SREBP1c expression, we treated liver carcinoma–derived Huh7 cells with a panel of oxysterols selected among the most abundant in the bloodstream, including 4β-, 7β-, 19-, 20-, 24(S)-, 25-, and 27-HC. The gene discussed is SREBF1; the disease is hepatocellular carcinoma.