PRSS50 and hepatocellular carcinoma: To analyse the role of TSP50 in hepatic carcinoma cells, pcDNA3.1‐TSP50 and PE‐GFP‐TSP50 were transfected into L02 cell for TSP50 overexpression, pSGU6/GFP/Neo‐shTSP50 were transfected into Huh7 cell and Bel7402 cell for TSP50 knockdown, and efficient TSP50 overexpression and knockdown were confirmed by Western blotting (Figure 1B‐F) (Figure S1B‐F).