To explore the functional role of TSP50 in hepatic carcinoma cells, we first detected the expression of TSP50 in human embryonic hepatocytes (L02 cell) and various HCC cell lines (SMMC‐7721 cell, Huh7 cell, HepG2 cell and Bel7402 cell). The gene discussed is PRSS50; the disease is hepatocellular carcinoma.