CD4 and infection: In contrast to that of TIGIT, even at 7 days after infection, the expression of CD226 on T cells was hardly affected by T. gondii infection (CD226+CD4+ T cells among PBMCs: 27.31% ± 5.671% vs. 31.74% ± 4.430%, p = 0.48; CD226+CD8+ T cells among PBMCs: 93.28% ± 3.949% vs. 86.42% ± 6.86%, p = 0.16; CD226+CD4+ T cells in the spleen: 32.53% ± 3.249% vs. 28.80% ± 3.73%, p = 0.31; CD226+CD8+ T cells in the spleen: 84.03% ± 0.725% vs. 83.31% ± 2.275%, p = 0.77).