All these results strongly suggested that VEGF-D/VEGFR-3 signaling is elevated in the reactive astrocytes and plays a crucial role in ischemic stroke-induced formation of astrogliosis and glial scar and knockdown of RIP1K can block the VEGF-D/VEGFR-3 signaling-induced astrogliosis and glial scar formation. The gene discussed is VEGFD; the disease is ischemic stroke.