ASCL2 and neoplasm: Based on the statistical method in TIMER database, we found the expression of ASCL2 and ETV4 had prominent anticorrelation with CD8+ scores (R_ASCL2 = −0.44 and R_ETV4 = −0.41) (Figure 3(b)), which indicated that increased ASCL2 and ETV4 might be associated with the decreased abundance of CD8+ T cells in the MSS tumor microenvironment.