In this study, by analysis of overall survival of patients with HNSCC by the Kaplan-Meier curve, we noticed that the glycolysis-related prognostic risk markers composed of HPRT1, STC2, PLCB3, GPR87, PYGL, and SLC5A12 can be applied as a predictive biomarker for the prognosis of HNSCC patients. This evidence concerns the gene PYGL and head and neck squamous cell carcinoma.