To evaluate the effect of the mTOR inhibitors temsirolimus and everolimus on neuroendocrine tumor cells, five NEN cell lines from different organs of origin were studied: BON and QGP-1 (both from pancreas), LCC-18 (from colon), and H727 and UMC-11 (both from lung). This evidence concerns the gene MTOR and neuroendocrine neoplasm.