Low ANO7 expression was linked to advanced tumor stage (P < 0.0001), high classical and quantitative Gleason grade (P < 0.0001 each), presence of lymph node metastasis (P < 0.0001), high preoperative PSA level (P < 0.0001), and positive surgical margin (P < 0.0001; Table 1). This evidence concerns the gene ANO7 and metastatic malignant neoplasm in the lymph nodes.