In addition, knockdown of BYSL significantly decreased the cell viability in primary glioma cells at 24 h (GBM-01: P = 0.002; GBM-03: P < 0.001), 48 h (GBM-01: P = 0.011; GBM-03: P = 0.005), and 72 h (GBM-01: P < 0.001; GBM-03: P = 0.004) (Figure 3F). The gene discussed is BYSL; the disease is central nervous system cancer.