BYSL and central nervous system cancer: Different concentrations of siRNAs were used to transfect glioma cells, and qRT-PCR assays showed that 20 nM, 50 nM, and 100 nM siRNA significantly reduced the BYSL mRNA levels in U251 (all, P < 0.001) and U87 (all, P < 0.001) cells (Figure 2A).