PYCARD and Familial prostate cancer: Interestingly, methylation status of PYCARD promoter was significantly associated with Gleason score (P = 0.0063) or Grade Group (P = 0.002): prostate cancers with Gleason score ≥ 7 (Grade Group ≥ 2) but not Gleason score 6 (Grade Group 1) showed tumor-specific hypermethylation (Gleason score ≥ 7: 96%, 44/46, Gleason score 6: 25%, 1/4).