After the glial ablation model of depression was developed [18], the antidepressant-like effect of mGluR5 antagonist MTEP [38], prefrontal cortex deep brain stimulation [39], Y5 receptor antagonist Lu AA33810 [40], Harmine [41], ZL006 [42], and ginsenoside Rf [43] were tested. This evidence concerns the gene GRM5 and major depressive disorder.