A study by Zhang et al. [53] revealed that cinobufagin, which has a similar structure with cinobufotalin, can inhibit U2OS/MG-63 spheroid/mother cell survival in a time- and dose-dependent manner; and the tumorigenesis capability of osteosarcoma cells can be inhibited by blocking the IL-6-OPN-STAT3 signaling pathway. This evidence concerns the gene SPP1 and osteosarcoma.