Our research showed that expression of TLR4, NF-κB, and MyD88 was significantly, markedly increased by RWIS in the model group and significantly decreased by pretreatment with electroacupuncture, indicating that preventive electroacupuncture could inhibit the activation of TLR4/NF-κB signaling induced by stress ulcer. Here, TLR4 is linked to duodenal ulcer.