To extend our observations to chronic liver damage wild-type and Trem-2 KO mice were exposed to carbon tetrachloride (CCl4) treatment for 4 weeks and analyzed on day 1 (D1) and day 3 (D3) after treatment corresponding to established liver fibrosis and fibrosis regression time points, respectively (Figure 3A). This evidence concerns the gene TREM2 and Hepatic fibrosis.