The present study focused on the relation of SNPs of miRNA involved in the insulin signaling pathways, miR-133a-1 rs8089787, miR-133a-2 rs13040413, let-7a-1 rs13293512, let-7f rs10877887, and miR-27a rs895819, with T2DM, and the interaction effects of SNP-SNP and SNP-environmental factors on T2DM, as well as the effect of the risk-associated polymorphism on regulating its mature miRNA expression. This evidence concerns the gene INS and type 2 diabetes mellitus.