As above, in our results, the expression of TH (as a classical marker of presynaptic sympathetic innervation) in the longitudinal smooth muscle of the urethral section in the DM group was significantly increased, and the expression of nNOS in DM group was significantly decreased, resulting in the imbalance of muscle coordination of the urinary function in the DM group causing DBD and urinary incontinence. The gene discussed is NOS1; the disease is Urinary incontinence.