When EC cases were classified by histological type, in ESCC patients (n=8) circulating ENO1 concentrations did not differ from those in normal subjects (mean±SE pg/ml: 2849±309 vs 3693±246, p=0.083), but were significantly higher than in EAC patients (n=24) (mean±SE pg/ml: 2849±309 vs 1961±185, p=0.022). Here, ENO1 is linked to esophageal squamous cell carcinoma.