To test whether anti-CD105 CAR-T cells could specifically kill CD105+ target cells, the Utd, Mock, anti-CD19 CAR-T cells, and anti-CD105 CAR-T cells were challenged with PKH26-labeled HCC cells (Bel7404, SMMC7721, HepG2, or MHCC97H), HUVEC, and 293T cells at E/T ratios of 1 : 3, 1 : 1, and 3 : 1 for 16 h. This evidence concerns the gene ENG and hepatocellular carcinoma.