Finally, to investigate whether the defecation stimulation effects of TEE are associated with the regulation of the mAChR signaling pathway in transverse colon of constipation rats, alterations in the expressions of mAChR M2, mAChR M3, Gα, PKC, p-PKC, PI3K and p-PI3K were measured in the transverse colons of subset groups. Here, PRRT2 is linked to Constipation.