To investigate whether the antioxidant activity of TEE in pRISMCs can be completely reflected into the regulation of oxidative defense in transverse colon of constipation rats, we measured alterations in the mRNA, protein and activity level of SOD, Nrf2 expression, CAT activity, and ROS level in the transverse colon of the Lop-induced constipation model after TEE treatment. The gene discussed is CAT; the disease is constipation disorder.