BDNF and spinocerebellar ataxia 7: After 14 days culture in neuronal differentiation medium containing N2/B27 supplement, cAMP ascorbic acid, BDNF and GDNF [25], both SCA7 patient and control NPCs stained positive for the neuronal marker βIII-Tubulin, and showed robust, diffuse nuclear localisation of the disease-causing protein, ATXN7 (Fig 1E and 1F).