To the best of our knowledge, there has been no previous study reporting the Se effects on cell viability, caspase 3 and caspase 9 activities, apoptosis rates, intracellular ROS and JC-1 values, and procaspase 3 and procaspase 9 and poly (ADP-ribose) polymerase 1 (PARP1) expression levels through the TRPV1 channel in human DBTRG glioblastoma cells over the evaluation of toxicity of dental amalgams containing Hg. This evidence concerns the gene CASP3 and glioblastoma.