To examine the effect of FBXO1 in breast cancer cell lines, MCF7 and MDA-MB-231 were successfully transfected with si‐FBXO1 to knockdown expression of FBXO1 and verified by Real-time qPCR, Western-blot analysis and FAM-fluorescence detection (Fig. 8b–d). This evidence concerns the gene CCNF and breast cancer.