To clarify the biological effect of TRIM47 in RCC, we firstly detected the expression of TRIM47 in 6 different RCC cell lines and normal renal cell HK2 by using Western blot and qRT-PCR, and the results showed that 769P and A498 cells highly expressed TRIM47 while 786-O and ACHN cells lowly expressed TRIM47 (Fig. 2a, b).Then the overexpression-TRIM47 cells (786-O,ACHN) and knockdown-TRIM47 cells(769P,A498) were constructed and observed by fluorescence microscope and Western blot (Fig. 2c, d). This evidence concerns the gene TRIM47 and renal cell carcinoma.