Furtherly, the protein interaction between TRIM47 and P53 in RCC 769-P cell line was detected by co-immunoprecipitation assay, and the result showed that the His antibody can pull out His-P53 itself, but the IgG does not pull out the band, indicating that the IP experiment was successful and there was no false positive. The gene discussed is TP53; the disease is renal cell carcinoma.